通过表型与基因组分析解析 Limosilactobacillus reuteri S8 在肉鸡中的益生菌潜力

• Raana Tabashiri¹,
• Sahar Mahmoodian²,
• Mohammad Hossein Pakdel³,
• Amir Meimandipour² &
• …
• Javad Zamani Amirzakaria⁴ 

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Abstract

The ban on antibiotic growth promoters in poultry has increased the need for probiotics. This study evaluates Limosilactobacillus reuteri S8, isolated from the gastrointestinal tract of broiler chickens, using in vitro assays, whole-genome sequencing and comparative pan-genomics. The strain showed a log-based survival rate of 85% at pH 3.0 (a 1.2 log₁₀ reduction in viable count over 3 h) and a relative growth of 50.5% in 0.3% bile salts, consistent with the presence of the atp operon and nhaC. It adhered to Caco-2 cells more densely than a low-adhesion reference strain, a favourable prerequisite for potential competitive exclusion that warrants further verification in vivo, and the genome encodes a sortase A homologue, a putative adhesin and exopolysaccharide biosynthesis genes. Under the in vitro conditions tested, it displayed antagonistic activity against all indicator organisms, including reference strains of Escherichia coli ATCC 25,922 and Salmonella enterica ATCC 14,028, consistent with multiple lactate dehydrogenase variants and a putative Class III bacteriocin locus. Pan-genome analysis with 62 reference genomes identified 155 strain-specific gene clusters, mainly associated with cellular biogenesis and DNA maintenance. The strain was resistant to tetracycline and ampicillin, and the genome carries the acquired antimicrobial resistance genes (ARGs) tet(W), tet(L) and lnu(A); tet(L) lies next to a recombinase gene (xerC), which warrants investigation of its possible mobility. Therefore, while commercial application is cautioned against due to the presence of acquired ARGs, this strain remains a valuable biological model for investigating antimicrobial resistance reservoirs in avian commensals.

Acknowledgements

This study with the project number of 764 has been approved by the national institute of genetic engineering and biotechnology.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

1. Agricultural Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

   Raana Tabashiri

2. Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, 84 8415683111, Iran

   Sahar Mahmoodian & Amir Meimandipour

3. Department of Plant Molecular Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

   Mohammad Hossein Pakdel

4. Department of System Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

   Javad Zamani Amirzakaria

Authors

1. Raana Tabashiri
2. Sahar Mahmoodian
3. Mohammad Hossein Pakdel
4. Amir Meimandipour
5. Javad Zamani Amirzakaria

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Correspondence to Amir Meimandipour.

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Competing interests

The authors declare no competing interests.

Ethics

All animal handling procedures adhered to protocols approved by the Institute of Animal Care and Use Committee at the National Institute of Genetic Engineering and Biotechnology, aligning with relevant national animal welfare guidelines and regulations.

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Tabashiri, R., Mahmoodian, S., Pakdel, M.H. et al. Deciphering the probiotic potential of Limosilactobacillus reuteri S8 in broilers through phenotypic and genomic analyses. Sci Rep (2026). https://doi.org/10.1038/s41598-026-77343-6

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• Received: 10 July 2026

• Accepted: 19 October 2026

• Published: 27 October 2026

• DOI: https://doi.org/10.1038/s41598-026-77343-6

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